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    Union Catalogue of Agricultural Libraries in the Netherlands

    The WUR Library Catalogue contains bibliographic data on books and periodicals held by the libraries of Wageningen University and Research Centre and some 15 associated libraries. Holding data are added to each record.

    Subjects covered include Agrotechnology, Food and Food Production, Plant and Animal Sciences, Soil Science, Geo-information, Landscape and Spatial Planning, Water and Climate, Ecosystem Studies, Economics and Society.

    The joint collections of the participating libraries cover a substantial part of the internationally available scientific literature in these disciplines.

    As far as Dutch scientific literature in these fields is concerned, coverage can be considered near 100%, including much of the so-called "grey literature".

    All titles are entered in their original language. Keywords are added to facilitate subject searching.

    The database is updated every day and now contains over 830.000 records.

Record number 104073
Title The permeability of dead plant cells for some enzymes
show extra info.
door N. Gorin
Author(s) Gorin, N.
Publisher Wageningen : Veenman
Publication year 1969
Description 93 p
Notes Proefschrift Wageningen
Ook verschenen als handelsuitgave
Tutors Hartog, Prof. Dr. C. den
Graduation date 1969-03-11
Dissertation no. 442
Author abstract show abstract

The penetration of α-chymotrypsin and/or pancreatic lipase into dead cells of soybean cotyledons, of yeast and of algae was studied using the enzymic activity as a parameter. In addition a fluorescent antibody technique was applied for the localization of α-chymotrypsin within the soybean cells.

The digestibility of unheated, EDTA-treated substrate was similar to that of the heated substrate. Since EDTA-treatment increases the permeability of the cell wall and cell membrane of the plant cell without denaturing proteins contained in the cells, we concluded that heating affected mainly the barrier formed by the cell wall, thus permitting a better passage of big molecules (enzymes).

Leakage of β-amylase (mol. weight 61,700) from soybean occurred only after treatment with EDTA. This confirmed that EDTA enhances the permeability of the cell wall.

α-Chymotrypsin (mol. weight 24,000) and pancreatic lipase (mol. weight 38,000) penetrated unheated sections, whereas amylopectin (mol. weight 50,000-1,000,000) did not penetrate unheated cotyledons. Apparently the greater dimension of amylopectin compared with α-chymotrypsin and lipase accounts for its lack of entry into unheated soybean cotyledons. However, when we applied the fluorescent antibody technique to localize α-chymotrypsin within the cells from unheated sections, we used antibodies (γ-globulins) having a mol. weight of 150,000. They entered the unheated sections and produced a positive result of the experiment. Consequently the size of the molecules as indicated by the molecular weight, is not the reason why amylopectin did not penetrate the unheated cotyledons. Moreover, this shows that α-chymotrypsin probably opens a way for the entry of globulins. Therefore the entry of proteolytic enzymes is not an inert process. This is conceivable as the enzymes are big molecules with catalytic activity.

Unheated non-EDTA-treated soybean (with plasmodesmata) was slightly penetrated by α-chymotrypsin, whereas unheated non-EDTA-treated yeast and algae (both without plasmodesmata) were not penetrated at all. Furthermore, in soybean material (unheated or heated) α-chymotrypsin enhanced the penetration of lipase; this effect was absent in the case of yeast. The results obtained strongly suggest that plasmodesmata are a way in dead plant cells for penetration of enzymes.

Online full textINTERNET
On paper FORUM ; STACKS ; NN08200,442
FORUM ; STACKS ; NN08202,442
Keyword(s) (cab) membranes / transport / cytology / plant physiology / enzymes / enzymology / fermentation / cell physiology
Categories Membranes
Publication type PhD thesis
Language English
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