Library Catalogue

Library Catalogue

  • external user (warningwarning)
  • Log in as
  • About the Catalogue

    Union Catalogue of Agricultural Libraries in the Netherlands

    The WUR Library Catalogue contains bibliographic data on books and periodicals held by the libraries of Wageningen University and Research Centre and some 15 associated libraries. Holding data are added to each record.

    Subjects covered include Agrotechnology, Food and Food Production, Plant and Animal Sciences, Soil Science, Geo-information, Landscape and Spatial Planning, Water and Climate, Ecosystem Studies, Economics and Society.

    The joint collections of the participating libraries cover a substantial part of the internationally available scientific literature in these disciplines.

    As far as Dutch scientific literature in these fields is concerned, coverage can be considered near 100%, including much of the so-called "grey literature".

    All titles are entered in their original language. Keywords are added to facilitate subject searching.

    The database is updated every day and now contains over 830.000 records.

Record number 104484
Title Potato leafroll virus, its purification from its vector Myzus persicae
show extra info.
door D. Peters
Author(s) Peters, D.
Publisher Wageningen : Veenman
Publication year 1967
Description 100 p
Series title Publicatie / Stichting Fonds Landbouw Export Bureau 1916/1918 (45)
Series title Mededeling / Laboratorium voor Entomologie (00142)
Series title Mededeling / Laboratorium voor Virologie (47)
Notes Proefschrift Wageningen
Tutors Want, Prof. Dr. Ir. J.P.H. van der ; Wilde, Prof. Dr. J. de
Graduation date 1967-10-06
Dissertation no. 414
Author abstract show abstract
Experiments were described on the purification of potato leafroll virus (PLRV) from its vector Myzus persicae. Preliminary experiments demonstrated that much non- viral material had to be removed from aphid macerates to obtain a pure virus preparation. This was achieved by a multistep procedure, in which the aphid macerates were emulsified with chloroform at pH 5.0. The virus present in the interphase between the chloroform and water phases was extracted from the interphase, and concentrated by high-speed centrifuging. The virus suspension obtained was subjected to a phase system of butoxy-ethanol, ethoxy-ethanol and 2.5 M phosphate buffer pH 7.5, and centrifuged in a sucrose density gradient column.

A fairly pure virus preparation was obtained when the material from the infective zone of the gradient was concentrated at 90,000 g for 3 h. Particles with a diameter of 23 mμand with a hexagonal outline were found in each preparation by electron- microscopy. The PLRV isolate purified was free of any contaminating virus when its biological purity was tested. One of the PLRV isolates used in the preliminary experiments, was contaminated with other, presumable persistent, viruses. Their properties and some studies on their host range were described. These viruses were referred to as virus-like particles.

Online full textINTERNET
On paper FORUM ; STACKS ; NN08200,414
FORUM ; STACKS ; NN08202,414
FORUM ; STACKS ; NN00656,45
FORUM ; STACKS ; NN08397,47
Keyword(s) (cab) plant diseases / plant viruses / solanum tuberosum / potatoes / plant protection / destruction / disease vectors / insects / vector control
Categories Plant Viruses / Potatoes
Publication type PhD thesis
Language English
About cum laude graduation (with distinction)
There are no comments yet. You can post the first one!
Post a comment

To support researchers to publish their research Open Access, deals have been negotiated with various publishers. Depending on the deal, a discount is provided for the author on the Article Processing Charges that need to be paid by the author to publish an article Open Access. A discount of 100% means that (after approval) the author does not have to pay Article Processing Charges.

For the approval of an Open Access deal for an article, the corresponding author of this article must be affiliated with Wageningen University & Research.

Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.