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    Union Catalogue of Agricultural Libraries in the Netherlands

    The WUR Library Catalogue contains bibliographic data on books and periodicals held by the libraries of Wageningen University and Research Centre and some 15 associated libraries. Holding data are added to each record.

    Subjects covered include Agrotechnology, Food and Food Production, Plant and Animal Sciences, Soil Science, Geo-information, Landscape and Spatial Planning, Water and Climate, Ecosystem Studies, Economics and Society.

    The joint collections of the participating libraries cover a substantial part of the internationally available scientific literature in these disciplines.

    As far as Dutch scientific literature in these fields is concerned, coverage can be considered near 100%, including much of the so-called "grey literature".

    All titles are entered in their original language. Keywords are added to facilitate subject searching.

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Record number 104673
Title Computer simulation of transport patterns of associating macromolecules : with special attention to the electrophoresis of αS1- and β-casein
show extra info.
H. Nijhuis
Title-variation: Computer simulation of transport patterns of associating macromolecules : with special attention to the electrophoresis of [alpha]S1- and [beta]-casein
Author(s) Nijhuis, H.
Publisher Wageningen : Veenman
Publication year 1974
Description 56 p
Notes Proefschrift Wageningen
Ook verschenen als handelsuitgave
Tutors Veeger, Prof. Dr. C.
Graduation date 1974-01-16
Dissertation no. 575
Author abstract show abstract

The anomalous boundaries occurring during free electrophoresis or ultracentrifugation of interacting biopolymers are investigated. As is already known from the early studies of LONGSWORTH and MACINNES (1942), such interactions can give rise to abnormal velocities and areas of the migrating peaks in transport patterns.

In this thesis in particular the complex formation between α S1 - and β-casein two major proteins from milk, was studied. The importance of such complex formation for the cohesion of the natural casein micelles in milk has been stressed repeatedly.

Complex formation between these proteins was easily recognizable from the different number of moving peaks on both sides of the electrophoretic channel.

Application of the moving boundary theory (Chapter 2) leads to the conclusion that the constituent mobility of α S1 -casein is fairly high, which indicates the presence of complexes of a high stoichiometric ratio α S1 /β.

The development of the reaction boundaries during electrophoresis was simulated in the ALGOL-programs presented in Chapter IV. In the computations the self-polymerization of α S1 -casein under the experimental conditions and the simultaneous formation of the various complexes was taken into account. The results of such a simulation was found to be consistent with the conclusions of the moving boundary theory.

The simulation (Chapter 3) is brought about by dividing the electrophoretic channel into a large number of small boxes of equal length. The development of the reaction boundary then was simulated by means of alternate rounds of transport of material from one box to the next and subsequent re-equilibration. Only the transport due to the velocity is accounted for, which makes the procedure a simplification of GOAD's method for the numerical solution of the conservation-of- mass equation of a system of migrating and interacting macromolecules. By reducing the calculation in this way a diffusion-like error is introduced which is used to advantage to imitate the diffusional flux. It is shown that this procedure is essentially identical to the countercurrent analog developed by BETHUNE and KEGELES to account for the effect of diffusion on the transport pattern of interacting proteins.

The adjustment of the simulated diffusion coefficients to their actual values is discussed and the results of different calculations compared. It is shown that in agreement with expectation diffusional spreading had only a minor influence on the development of a reaction boundary in prolonged experiments.

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On paper FORUM ; STACKS ; NN08202,575
FORUM ; STACKS ; NN08200,575
Keyword(s) (cab) electrophoresis / phosphoproteins / casein / computer simulation / numerical methods
Categories Biochemistry
Publication type PhD thesis
Language English
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