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Library Catalogue

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    Union Catalogue of Agricultural Libraries in the Netherlands

    The WUR Library Catalogue contains bibliographic data on books and periodicals held by the libraries of Wageningen University and Research Centre and some 15 associated libraries. Holding data are added to each record.

    Subjects covered include Agrotechnology, Food and Food Production, Plant and Animal Sciences, Soil Science, Geo-information, Landscape and Spatial Planning, Water and Climate, Ecosystem Studies, Economics and Society.

    The joint collections of the participating libraries cover a substantial part of the internationally available scientific literature in these disciplines.

    As far as Dutch scientific literature in these fields is concerned, coverage can be considered near 100%, including much of the so-called "grey literature".

    All titles are entered in their original language. Keywords are added to facilitate subject searching.

    The database is updated every day and now contains over 830.000 records.

Record number 104736
Title Characterization of pectin lyases on pectins and methyl oligogalacturonates
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A.G.J. Voragen
Author(s) Voragen, A.G.J.
Publisher Wageningen : Pudoc
Publication year 1972
Description XIV, 121 p
Notes Proefschrift Wageningen  show all notes
Met samenvatting in het Nederlands
Ook verschenen als handelsuitgave
ISBN 9022004090
Tutors Pilnik, Prof. Dr. W.
Graduation date 1972-10-25
Dissertation no. 530
Author abstract show abstract

Pure saturated and unsaturated oligogalacturonic acids, including unsaturated monogalacturonic acid, were isolated and characterized. Their ultraviolet absorbance at 232 run, and their reactivities in the periodate thiobarbituric acid test and carbazole tests were studied. From these compounds methyl oligogalacturonates were prepared, which were used as model substrates.

Pectin lyases (EC; poly-α-1,4-D-methyl-galacturonate lyase) were found to be very common in commercial 'pectinase' preparations. From each of three 'pectinase' preparations a pectin lyase was isolated free of other pectolytic enzymes. Their pH optimum, pH stability, isoelectric point and activation energy were determined. Information on the action of the enzymes on pectins with various degrees of esterification ( DE ) was obtained by kinetic studies, by determining the extent of degradation and by analysing the composition of the reaction mixtures. The breakdown mechanism on pectin and pattern of action on methyl oligogalacturonates was studied. Divalent cations were found to activate pectin lyase activity, their activation depended on the pH and DE. Literature on the isolation and characterization of oligogalacturonides and on pectin degrading enzymes is reviewed.

Online full textINTERNET
On paper FORUM ; STACKS ; NN08202,530
FORUM ; STACKS ; NN08200,530
Keyword(s) (cab) lyases / pectins
Categories Postharvest Treatment / Proteins and Enzymes
Publication type PhD thesis
Language English
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