Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 120974
Title Modelling the effect of temperature and pH on the activity of enzymes: the case of phytases.
Author(s) Tijskens, L.M.M.; Greiner, R.; Biekman, E.S.A.; Konietzny, U.
Source Biotechnology and Bioengineering 72 (2001)3. - ISSN 0006-3592 - p. 323 - 330.
Department(s) Horticultural Supply Chains
Publication type Refereed Article in a scientific journal
Publication year 2001
Abstract In this study, the behavior of enzyme activity as a function of pH and temperature is modeled on the basis of fundamental considerations. A formulation is developed that includes the activation of enzymes with increasing temperatures and the deactivation of enzymes at higher temperature, together with the effect of protonation and hydroxylation on activity at various constant pH levels. The model is calibrated and validated against an extensive set of experimental data on phytases from seven different origins. The percentage variance accounted for (R), obtained by statistical nonlinear regression analysis on all data sets, was shown to range from 97.6␝o 99.5ÐThe equilibrium constant of protonation and hydroxylation proved to be independent of temperature
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