Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 12256
Title Nucleotide sequence of the regulatory nifA gene of Rhizobium leguminosarum PRE: transcriptional control sites and expression in Escherichia coli.
Author(s) Roelvink, P.W.; Hontelez, J.G.J.; Kammen, A. van; Bos, R.C. van den
Source Molecular Microbiology 3 (1990)10. - ISSN 0950-382X - p. 1441 - 1447.
Department(s) Laboratory of Virology
Laboratory of Molecular Biology
Publication type Refereed Article in a scientific journal
Publication year 1990
Abstract We report the sequence of the regulatory nifA gene of Rhizobium leguminosarum PRE. The transcription initiation and termination sites of nifA were mapped and a potential promoter and a rho-independent terminator identified. The nifA gene has two possible translation start sites, both of which are used in an Escherichia coli background, resulting in proteins with apparent molecular weights of 58kD and 57kD; initiation at the second site is preferred over initiation at the first. The nifA-nifB intergenic region contains an rpoN-dependent promoter for the nifB gene but no consensus upstream activator sequence (UAS).
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