Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 122763
Title AFLPs reveal genomic regions not detected by RFLPs: a case study in tomato
Author(s) Bonnema, G.; Berg, P. van den; Lindhout, P.
Source Genome 45 (2002). - ISSN 0831-2796 - p. 217 - 221.
DOI http://dx.doi.org/10.1139/g01-145
Department(s) Laboratory of Plant Breeding
EPS
Publication type Refereed Article in a scientific journal
Publication year 2002
Abstract A set of three tomato chromosome 7 introgression lines (ILs) containing overlapping segments of Lycopersicon pennellii DNA was screened with a set of 10 EcoRI–MseI and 10 PstI–MseI AFLP primer combinations. A large number of markers were identified that mapped to one of the four regions of chromosome 7 defined by the set of three ILs. Because many of the identified markers have known map positions in three tomato reference maps, their location on the tomato genome could be verified. It was demonstrated that the three chromosome 7 ILs carried a chromosome 10 region harbouring a cluster of six AFLP markers that had not been detected before using RFLPs. The causes and implications of this observation are discussed.
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