Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 302989
Title Regulation of the inductive phase of microspore embryogenesis in Brassica napus
Author(s) Custers, J.B.M.; Cordewener, J.H.G.; Dons, H.J.M.; Lookeren Campagne, M.M. van
Source Acta Horticulturae 407 (1996). - ISSN 0567-7572 - p. 209 - 217.
Department(s) Centrum voor Plantenveredelings- en Reproduktieonderzoek
Publication type Refereed Article in a scientific journal
Publication year 1996
Abstract In cultured microspores from Brassica napus, embryogenesis can be synchronously and irreversibly induced by elevating the culture temperature to 32°C for a minimum of 8 h. Culture at 18°C allows gametophytic development to continue, and results in the formation of pollen in vitro. This allows us to study the temperature controlled switch in developmental fate from gametophytic development to embryogenic development by molecular means. Analysis of protein synthetic patterns by [35S]-methionine incorporation and 2-dimensional gel electrophoresis, revealed that 25 proteins were differentially synthesized during the induction of microspore embryogenesis. Most of these proteins (17) appeared to belong to the class of heat shock proteins (HSPs). Four of these HSPs have been identified by Western blotting using antibodies raised against HSP17, HSP68 and HSP70. One protein that was only synthesized under embryogenic culture conditions, and did not belong to the heat inducible HSPs, is a candidate marker for early embryogenic development.
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