Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 303859
Title Identification and characterization of potyviruses of Alstroemeria spp
Author(s) Bouwen, L.; Vlugt, R.A.A. van der
Source Acta Horticulturae 432 (1996). - ISSN 0567-7572 - p. 72 - 74.
Department(s) Research Institute for Plant Protection
Publication type Refereed Article in a scientific journal
Publication year 1996
Abstract Several viruses have been reported to infect Alstroemeria. The two most important ones are considered to be Alstroemeria mosaic (AIMV) and Alstroemeria streak virus (AISV). Both AIMV and AISV react in ELISA with a commonly used antiserum to AIMV. There are indications that potyviruses other than AIMV and AISV also occur in Alstroemeria. The present study was initiated to identify and characterise these potyviruses. A strategy was developed that is aimed at separation of these viruses and their subsequent individual characterization. It is based on obtaining isolated of single viruses by serial passages through a local lesion host followed by RT-PCR or immuno-capture RT-PCR to obtain virus-specific cDNA fragments. The nucleotide sequence of these cDNA fragments is compared to those of other known potyviruses present in the EMBL Database. Using this identification strategy four different potyviruses were identified, three of which were not known to occur in Alstroemeria.
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