Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 305080
Title Embryogenesis from isolated microspores of tulip : towards developing F1 hybrid varieties
Author(s) Custers, J.B.M.; Ennik, E.; Eikelboom, W.; Dons, J.J.M.; Lookeren Campagne, M.M. van
Source Acta Horticulturae 430 (1997). - ISSN 0567-7572 - p. 259 - 266.
Department(s) Centrum voor Plantenveredelings- en Reproduktieonderzoek
Publication type Refereed Article in a scientific journal
Publication year 1997
Abstract This report describes advances in the technique of embryogenesis from tulip microspores in culture. High temperature pretreatment (32°C) of bulbs, which contain fully developed inflorescences, had a positive effect on the production of microspore embryos. The pretreatment shortened the time for embryo formation and stimulated the progression from the multicellular microspore stage into the embryo proper. Early unicellular microspores were found to be better starting material for culture than mid unicellular microspores. The technique of microspore embryogenesis was applicable to various tulip cultivars. The improved protocol now allows the production of large numbers of microspore-derived plants, and the further evaluation of doubled haploid plant technology for tulip.
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