Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 306177
Title Detection and identification of Phytophthora fragariae Hickman by the polymerase chain reaction
Author(s) Bonants, P.; Hagenaar-de Weerdt, M.; Gent-Pelzer, M. van; Lacourt, I.; Cooke, D.; Duncan, J.
Source European Journal of Plant Pathology 103 (1997). - ISSN 0929-1873 - p. 345 - 355.
Department(s) Research Institute for Plant Protection
Publication type Refereed Article in a scientific journal
Publication year 1997
Abstract Phytophthora fragariae Hickman, which causes strawberry red stele and raspberry root rot, is a quarantine organism for which specific and sensitive detection methods are required to test the health of planting material. Sequences of the internal transcribed spacer regions of the ribosomal gene repeat (rDNA) were used to develop primers for P. fragariae in a nested Polymerase Chain Reaction (PCR). The fungus was readily detected in infected but symptomless roots by nested, but not single-round, PCR. It was also detected in infested water samples obtained from the Dutch General Inspection Service by nested PCR. Detection of PCR products was at least 10-fold more sensitive by PCR-ELISA than by conventional visualisation on agarose gels.
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