Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 315628
Title Biased chloroplast and mitochondrial transmission in somatic hybrids of Allium ampeloprasum L. and Allium cepa L
Author(s) Buiteveld, J.; Kassies, W.; Geels, R.; Lookeren Campagne, M.M. van; Jacobsen, E.; Creemers-Molenaar, J.
Source Plant Science 131 (1998)2. - ISSN 0168-9452 - p. 219 - 228.
Department(s) Centrum voor Plantenveredelings- en Reproduktieonderzoek
Institute for Forestry and Nature Research
Publication type Refereed Article in a scientific journal
Publication year 1998
Abstract Somatic hybrid plants, produced by symmetric protoplast fusion between leek (Allium ampeloprasum) and cytoplasmic male sterile onion (Allium cepa), were analysed for their organelle composition. Mitochondrial DNA (mtDNA) analyses were performed using PCR amplification of the V7 region of the mitochondrial small ribosomal RNA (srRNA) gene in conjunction with restriction site analysis and southern hybridization. Of the 55 hybrids analysed with the PCR method only three hybrids had mtDNA restriction fragment patterns of both parents, while the remaining hybrids displayed a pattern identical to A. ampeloprasum. Detailed analysis of 18 of these hybrids with southern hybridization using five mitochondrial probes revealed that 15 hybrids possessed a rearranged mitochondrial genome of both parents, but with a predominance of mtDNA fragments of A. ampeloprasum. The other three hybrids had the same hybridization pattern as A. ampeloprasum, indicating that these hybrids probably contain the unaltered parentalA. ampeloprasum mitochondrial genome. The same 18 hybrids were analysed for their cpDNA using the Petunia chloroplasts DNA pPCY64 probe. A total of 15 hybrids possessed chloroplasts identical to A. ampeloprasum and three had A. cepa chloroplasts. Therefore, it is suggested that the chloroplasts of the hybrids did not sort out randomly. Also a novel combination of chloroplasts and mtDNA was found in two hybrids. VA:IBN
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