Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 318510
Title Isolation and characterization of undenatured chlorogenic acid free sunflower (Helianthus annuus) Proteins
Author(s) Gonzales-Perez, S.; Merck, K.B.; Vereijken, J.M.; Koningsveld, G.A. van; Gruppen, H.; Voragen, A.G.J.
Source Journal of Agricultural and Food Chemistry 50 (2002). - ISSN 0021-8561 - p. 1713 - 1719.
Department(s) Agrotechnological Research Institute
Food Chemistry Group
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2002
Abstract A method for obtaining sunflower protein (SFP) isolate, nondenatured and free of chlorogenic acid (CGA), has been developed. During the isolating procedure, the extent of CGA removal and protein denaturation was monitored. The defatted flour contained 2.5 percent CGA as the main phenolic compound. Phenolic compounds were removed by aqueous methanol (80€extraction, before protein extraction at alkaline pH and diafiltration. Differential scanning calorimetry and solubility tests indicated that no denaturation of the proteins had occurred. The resulting protein products were biochemically characterized, and the presence of protein-CGA complexes was investigated. SFPs of the studied variety were found to be composed of two main protein fractions: 2S albumins and 11S globulins. In contrast to what has been previously reported, CGA was found to elute as free CGA, not covalently associated to any protein fraction.
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