Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 318529
Title Aggregation of peptides during hydrolysis as a cause of reduced enzymatic extractability of soybean meal proteins
Author(s) Fischer, M.; Gruppen, H.; Piersma, S.R.; Kofod, L.V.; Schols, H.A.; Voragen, A.G.J.
Source Journal of Agricultural and Food Chemistry 50 (2002). - ISSN 0021-8561 - p. 4512 - 4519.
Department(s) Food Chemistry Group
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2002
Abstract With the purpose of analyzing the size and composition of enzyme-unextractable proteins in differently heat-treated soybean meals, a selection of extractants was screened for their ability to extract these proteins from enzyme-unextractable residues. The largest effects were obtained with urea, urea plus -mercaptoethanol, and dilute alkali; the latter extracted up to 87␘f the enzyme-unextractable protein. Gel permeation chromatography indicated that a large proportion of the extracted material was of high molecular weight. However, the combined results from gel electrophoresis, LC-MS, and MALDI-ToF MS showed that the extracted protein material was composed of aggregated peptides. The largest aggregates were observed in the enzymatic residues originating from meals heat-treated at high humidity. Extracted aggregates were fully degraded upon subsequent proteolytic treatment
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