Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 318826
Title Assessment of the microbody luminal pH in the filamentous fungus Penicillium chrysogenum
Author(s) Lende, T.R. van der; Breeuwer, P.; Abee, T.; Konings, W.N.; Driessen, A.J.M.
Source Biochimica et Biophysica Acta. C, Molecular Cell Research 1589 (2002). - ISSN 0167-4889 - p. 104 - 111.
DOI https://doi.org/10.1016/S0167-4889(02)00162-3
Department(s) Food Microbiology Laboratory
VLAG
Publication type Refereed Article in a scientific journal
Publication year 2002
Abstract The enzymes of the penicillin biosynthetic pathway in Penicillium chrysogenum are located in different subcellular compartments. Consequently, penicillin pathway precursors and the biologically active penicillins have to cross one or more membranes. The final enzymatic step that is mediated by acyltransferase takes place in a microbody. The pH of the microbody lumen in penicillin producing cells has been determined with fluorescent probes and mutants of the green fluorescent protein and found to be slightly alkaline.
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