Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 320427
Title Biosensor immunoassay for the detection of eight sulfonamides in chicken serum
Author(s) Haasnoot, W.; Bienenmann-Ploum, M.; Kohen, F.
Source Analytica Chimica Acta 483 (2003)1-2. - ISSN 0003-2670 - p. 171 - 180.
Department(s) RIKILT - Business Unit Safety & Health
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) enzyme-immunoassay - sulfamethazine residues - 2 sulfonamides - antibodies - assay - immunobiosensor - sulfadiazine - extraction - tissues - kidney
Abstract A monoclonal antibody (MAb) raised against sulfamethazine (21C7) was applied in an optical biosensor (Biacore Q) to develop a rapid biosensor immunoassay (BIA) for the detection of several sulfonamides in chicken serum. The performance of this MAb was compared with two polyclonal antibodies (PAbs) raised against sulfamethazine (Qflex sulfamethazine binding protein (SBP) and RIKILT 464b). Using these PAbs, the limits of detection (LODs) in 10 times diluted chicken serum were approximately 30 ng ml-1 and the two BIAs were found to be specific for sulfamethazine. Using MAb 21C7, the LOD for sulfamethazine in 10 times diluted chicken serum was lower (10 ng ml-1), high cross-reactivities were measured for sulfisoxazole (149¿ sulfachlorpyridazine (112¿ sulfachlorpyrazine (94¿ sulfamerazine (87¿ sulfadiazine (56¿ sulfatroxazole (56¿and sulfathiazole (50¿and low cross-reactivities (11¿25¿were measured with six other sulfonamides. Compared with the polyclonal antibodies, the MAb-based BIA resulted in a better sensitivity and was found suitable for the detection of 8 sulfonamides in 10 times diluted chicken serum with LODs between 7 and 20 ng ml-1. The total run time for each cycle was 7 min.
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