Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 32058
Title Surfactant-induced non-lethal release of anthraquinones from suspension culture of Morinda citrifolia.
Author(s) Bassetti, L.; Hagendoorn, M.J.M.; Tramper, J.
Source Journal of Biotechnology 39 (1995)2. - ISSN 0168-1656 - p. 149 - 155.
Department(s) Laboratory of Plant Physiology
Sub-department of Food and Bioprocess Engineering
Publication type Refereed Article in a scientific journal
Publication year 1995
Abstract A new approach based on the use of the surfactant Pluronic F-68 to obtain non-lethal release of plant cell intracellular products was investigated. Suspension cultures of Morinda citrifolia (Rubiaceae), producing anthraquinones as secondary metabolites, were selected as model system. By supplementing the growth medium with 2% w/v of Pluronic F-68 for four repeated batch cycles, cells released anthraquinones to a concentration 170-times the value of the control cells. An average of 55% of the intracellularly stored anthraquinones was recovered from the Pluronic-treated cells, against 0.74% of the control. The determination of K+ leakage showed that cells did not release products through a diffusion mechanism due to membrane damages. Cell respiration was not affected by the presence of the surfactant. We suggest media supplementation with Pluronic as a simple, inexpensive and effective way to achieve non-lethal product release from plant cells.
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