Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 321005
Title Identification of an unknown Beta-agonist in feed by liquid chromatography/bioassay/quadrupole time-of-flight tandem mass spectrometry with accurate mass measurement
Author(s) Nielen, M.W.F.; Elliott, C.T.; Boyd, S.A.; Courtheyn, D.; Essers, M.L.; Hooijerink, H.H.; Bennekom, E.O. van
Source Rapid Communications in Mass Spectrometry 17 (2003)14. - ISSN 0951-4198 - p. 1633 - 1641.
DOI https://doi.org/10.1002/rcm.1099
Department(s) RIKILT - Analyse & OntwikkelingBU Microbiological & Chemical Food Analysis
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) urine - chromatography - online - clenbuterol - extraction
Abstract A new approach to the search for residues of unknown growth promoting agents such as anabolic steroids and -agonists in feed is presented. Following primary extraction and clean-up, samples are separated using gradient liquid chromatography (LC). The effluent is split towards two identical 96-well fraction collectors and an optional electrospray quadrupole time-of-flight mass spectrometry (QTOFMS) system for accurate mass measurement. One 96-well plate is used for a bioassay (enzyme-immuno assay, receptor assay) and will detect the bioactivity and position of the relevant peak in the chromatogram. The positive well in the second 96-well plate is used for identification by LC/QTOFMS/MS. The value of this LC/bioassay/QTOFMS/MS methodology is highlighted by the finding and structure elucidation of a new -agonist in a feed extract.
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