Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 327367
Title A method for the isolation of root hairs from the model legume Medicago truncatula
Author(s) Ramos Escribano, J.; Bisseling, T.
Source Journal of Experimental Botany 54 (2003)391. - ISSN 0022-0957 - p. 2245 - 2250.
DOI https://doi.org/10.1093/jxb/erg245
Department(s) Laboratory of Molecular Biology
EPS-1
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) symbiotic nitrogen-fixation - transformation - regeneration - expression - gene - proteins - plants
Abstract A new method for the isolation of root hairs from the model legume, Medicago truncatula, was developed. The procedure involves the propagation of detached roots on agar plates and the collection of root hairs by immersion in liquid nitrogen. Yields of up to 40 µg of root hair protein were obtained from 50–100 root tips grown for 3 weeks on a single plate. The high purity of the root hair fraction was monitored by western blot analysis using an antibody to the pea epidermis specific protein PsRH2. Sequence analyses revealed that the protein homologous to PsRH2 in M. truncatula, MtRH2, is identical to the root protein MtPR10‐1. The MtRH2 protein proved to be a useful endogenous marker to monitor root hair isolation since it is also specifically expressed in the root epidermis.
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