Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 327399
Title Isolation and characterization of two specific regulatory Aspergillus niger mutants shows antagonistic regulation of arabinan and xylan metabolism
Author(s) Groot, M.J.L. de; Vondervoort, P.J.I. van de; Vries, R.P. de; Kuyk, P.A. van; Ruijter, G.J.G.; Visser, J.
Source Microbiology 149 (2003). - ISSN 1350-0872 - p. 1183 - 1191.
DOI http://dx.doi.org/10.1099/mic.0.25993-0
Department(s) Microbiological Laboratory
Laboratory of Phytopathology
WU Agrotechnology and Food SciencesDepartment of Agrotechnology and Food Sciences
AFSG Staff Departments (WUATV)
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) alpha-l-arabinofuranosidase - transcriptional activator xlnr - gene-expression - d-xylose - degrading enzymes - degradation - induction - nidulans - cloning - construction
Abstract This paper describes two Aspergillus niger mutants (araA and araB) specifically disturbed in the regulation of the arabinanase system in response to the presence of L-arabinose. Expression of the three known L-arabinose-induced arabinanolytic genes, abfA, abfB and abnA, was substantially decreased or absent in the araA and araB strains compared to the wild-type when incubated in the presence of L-arabinose or L-arabitol. In addition, the intracellular activities Of L-arabitol dehydrogenase and L-arabinose reductase, involved in L-arabinose catabolism, were decreased in the araA and araB strains. Finally, the data show that the gene encoding D-xylulose kinase, xkiA, is also under control of the arabinanolytic regulatory system. L-Arabitol, most likely the true inducer of the arabinanolytic and L-arabinose catabolic genes, accumulated to a high intracellular concentration in the araA and araB mutants. This indicates that the decrease of expression of the arabinanolytic genes was not due to lack of inducer accumulation. Therefore, it is proposed that the araA and araB mutations are localized in positive-acting components of the regulatory system involved in the expression of the arabinanase-encoding genes and the genes encoding the L-arabinose catabolic pathway.
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