Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 327547
Title Solubilization and refolding of inclusion body proteins in reverse micelles
Author(s) Vinogradov, A.A.; Kudryashova, E.V.; Levashov, A.V.; Dongen, W.M.A.M. van
Source Analytical Biochemistry 320 (2003). - ISSN 0003-2697 - p. 234 - 238.
Department(s) Biochemistry
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) galactose-oxidase - escherichia-coli - crystal-structure - organic-solvents - enzyme - stabilization - bodies - water
Abstract Today, many valuable proteins can be obtained in sufficient amounts using recombinant DNA techniques. However, frequently the expression of recombinant proteins results in the accumulation of the product in dense amorphous deposits inside the cells, called inclusion bodies. The challenge then is to transform these inactive and misfolded protein aggregates into soluble bioactive forms. Although a number of general guidelines have been proposed, the search for proper reconstitution conditions can be very laborious and time consuming. Hare, we suggest a new versatile approach for solubilization and refolding of inclusion body proteins using a water-sodium bis-2-ethylhexyl sulfosuccinate-isooctane reverse micellar system. Instead of amorphous aggregates, a transparent solution is obtained, where refolded protein is entrapped inside the micelles. The entrapped enzyme has native-like secondary structure and catalytic activity. This approach has been implemented with Fusarium galactose oxidase and Stigmatella aurantiaca putative galactose oxidase. (C) 2003 Elsevier Science (USA). All rights reserved.
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