Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 327590
Title Cartilage Tissue Engineering: Controversy in the Effect of Oxygen
Author(s) Malda, J.; Martens, D.E.; Tramper, J.; Blitterswijk, C.A. van; Riesle, J.
Source Critical Reviews in Biotechnology 23 (2003)3-4. - ISSN 0738-8551 - p. 175 - 190.
Department(s) Bioprocess Engineering
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) human articular-cartilage - sensitive teratogen 3-acetylpyridine - intermittent hydrostatic-pressure - human nasal chondrocytes - chick limb - in-vitro - differentiated phenotype - vascularized mesenchyme - proteoglycan synthesis - dynamic compression
Abstract Articular cartilage lacks the ability to repair itself and consequently defects in this tissue do not heal. Tissue engineering approaches, employing a scaffold material and cartilage producing cells (chondrocytes), hold promise for the treatment of such defects. In these strategies the limitation of nutrients, such as oxygen, during in vitro culture are of major concern and will have implications for proper bioreactor design. We recently demonstrated that oxygen gradients are indeed present within tissue engineered cartilaginous constructs. Interestingly, oxygen, besides being an essential nutrient, is also a controlling agent of developmental processes including cartilage formation. However, the specific role of oxygen in these processes is still obscure despite the recent advances in the field. In particular, the outcome of published investigations is inconsistent regarding the effect of oxygen tension on chondrocytes. Therefore, this article describes the possible roles of oxygen gradients during embryonic cartilage development and reviews the data reported on the effect of oxygen tension on in vitro chondrocyte proliferation and differentiation from a tissue engineering perspective. Furthermore, possible causes for the variance in the data are discussed. Finally, recommendations are included that may reduce the variation, resulting in more reliable and comparable data.
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