Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 327980
Title Meiotic cohesin REC8 marks the axial elements of rat synaptonemal complexes before cohesins SMC1B and SMC3
Author(s) Eijpe, M.; Offenberg, H.H.; Jessberger, R.; Revenkova, E.; Heyting, C.
Source Journal of Cell Biology 160 (2003)5. - ISSN 0021-9525 - p. 657 - 670.
DOI https://doi.org/10.1083/jcb.200212080
Department(s) WU Plant SciencesDepartment of Plant Sciences
Laboratory of Genetics
EPS-4
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) sister-chromatid cohesion - schizosaccharomyces-pombe - mammalian smc1 - fission yeast - recombination proteins - chromosome dynamics - meiosis - localization - separation - cells
Abstract In meiotic prophase, the sister chromatids of each chromosome develop a common axial element (AE) that is integrated into the synaptonemal complex (SC). We analyzed the incorporation of sister chromatid cohesion proteins (cohesins) and other AE components into AEs. Meiotic cohesin REC8 appeared shortly before premeiotic S phase in the nucleus and formed AE-like structures (REC8-AEs) from premeiotic S phase on. Subsequently, meiotic cohesin SMC1beta, cohesin SMC3, and AE proteins SCP2 and SCP3 formed dots along REC8-AEs, which extended and fused until they lined REC8-AEs along their length. In metaphase 1, SMC1beta, SMC3, SCP2, and SCP3 disappeared from the chromosome arms and accumulated around the centromeres, where they stayed until anaphase II. In striking contrast, REC8 persisted along the chromosome arms until anaphase I and near the centromeres until anaphase II. We propose that REC8 provides a basis for AE formation and that the first steps in AE assembly do not require SMC1beta, SMC3, SCP2,, and SCP3. Furthermore, SMC1beta, SMC3, SCP2, and SCP3 cannot provide arm cohesion during metaphase II. We propose that REC8 then provides cohesion. RAD51 and/or DMC1 coimmunoprecipitates with REC8, suggesting that REC8 may also provide a basis for assembly of recombination complexes.
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