Staff Publications

Staff Publications

  • external user (warningwarning)
  • Log in as
  • language uk
  • About

    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 332603
Title Gene expression during anthesis and senescence in Iris flowers
Author(s) Doorn, W.G. van; Balk, P.A.; Houwelingen, A.M. van; Hoebrechts, F.A.; Hall, R.D.; Vorst, O.; Schoot, C. van der; Wordragen, M.F. van
Source Plant Molecular Biology 53 (2003). - ISSN 0167-4412 - p. 845 - 863.
DOI https://doi.org/10.1023/B:PLAN.0000023670.61059.1d
Department(s) AFSG Quality in Chains
PRI Bioscience
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) ribosome-inactivating proteins - programmed cell-death - shoot apical meristem - leaf senescence - salicylic-acid - regulated expression - molecular-cloning - petal senescence - plant defense - kinase ck2
Abstract We investigated changes in gene expression in Iris hollandicaflowers by microarray technology. Flag tepals were sampled daily, from three days prior to flower opening to the onset of visible senescence symptoms. Gene expression profiles were compared with biochemical data including lipid and protein degradation and DNA coiling, and with morphological data. Plasmodesmata of mesophyll cells closed about two days before flower opening, while in the epidermis they closed concomitant with opening. Similarly, the onset of visible senescence in the epidermis cells occurred about two days later than in the mesophyll. About 1400 PCR-amplified clones, derived from a subtractive cDNA library enriched for tepal-specific genes, were spotted and about 240 clones, including 200 that were expressed most differentially, were sequenced. The expression patterns showed three main clusters. One exhibited high expression during tepal growth (cluster A). These genes were putatively associated with pigmentation, cell wall synthesis and metabolism of lipids and proteins. The second cluster (B) was highly expressed during flower opening. The third cluster (C) related to the final stages of senescence, with genes putatively involved in signal transduction, and the remobilization of phospholipids, proteins, and cell wall compounds. Throughout the sampling period, numerous plant defence genes were highly expressed. We identified an ion channel protein putatively involved in senescence, and some putative regulators of transcription and translation, including a MADS-domain factor
Comments
There are no comments yet. You can post the first one!
Post a comment
 
Please log in to use this service. Login as Wageningen University & Research user or guest user in upper right hand corner of this page.