Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 332988
Title Novel pectate lyase gene from Globodera rostochiensis
Author(s) Kudla, U.; Qin Ling, ; Smant, G.
Source In: Nematology Symposium abstracts Leiden : Brill Academic Publishers - p. 222 (283) - 222 (283).
Department(s) Laboratory of Nematology
EPS-2
Publication type Abstract in scientific journal or proceedings
Publication year 2002
Abstract A novel cell wall degrading enzyme was identi¿ ed as a part of cDNA-AFLP project that compared gene expression pro¿ les in ¿ ve different stages of Globodera rostochiensis. One Transcript Derived Fragment (TDF), predominantly expressed in infective second stage juveniles, encoded a partial open reading frame showing high similarity to bacterial and fungal pectate lyases from the polysaccharide lyase 3 family (EC 4.2.2.2). The corresponding full-length cDNA designated Gr-Pel2 harbours an open reading frame of 759 bp, encoding polypeptide with molecular mass 27 kDa. Gr-Pel2 is signi¿ cantly different from previously identi¿ ed Gr- Pel1 (30% identities and 46% similarities). Sequence was predicted to have signal peptide for secretion at its N-terminal end. Digoxigenin-labelled DNA probe hybridised speci¿ cally to the subventral pharyngeal glands, which are responsible for secreting all currently known cell wall degrading enzymes. Heterologous expression of Gr-Pel2 in Pichia pastoris is in progress, in order to assess its biochemical properties. Identi¿ cation of another pectate lyase gene shows the complexity of the pectinolytic system in nematode secretions.
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