Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 338215
Title Measuring the Length Distribution of a Fibril System: a Flow Birefringence Technique applied to Amyloid Fibrils
Author(s) Rogers, S.S.; Venema, P.; Sagis, L.M.C.; Linden, E. van der; Donald, A.M.
Source Macromolecules 38 (2005)7. - ISSN 0024-9297 - p. 2948 - 2958.
Department(s) Physics and Physical Chemistry of Foods
Publication type Refereed Article in a scientific journal
Publication year 2005
Keyword(s) beta-lactoglobulin gels - rodlike chains subject - transient shear-flow - rheooptical response - carbon nanotubes - protein - macromolecules - polydispersity - disease - fibers
Abstract Relaxation of flow birefringence can give a direct measure of the rotational diffusion of rodlike objects in solution. With a suitable model of the rotational diffusivity, a length distribution can be sought by fitting the decay curve. We have measured the flow birefringence decay from solutions of amyloid fibrils composed of ß-lactoglobulin and extracted a length distribution using the Doi-Edwards-Marrucci-Grizzuti theory of semidilute rotational diffusion. The concentration scaling of the results shows that the fibrils diffuse as free rods: they cannot be significantly branched, sticky, or break up under dilution. The length distribution obtained shows a single broad peak, consistent with measurements of the fibrils by electron microscopy. This comparison, and combination of the experiment with an assay to find the total concentration of fibrils, allows calibration of the length scale and concentration scale of the length distribution. It is our hope that this method can be used for following the growth kinetics of amyloid fibrils in vitro and for studying the length distribution of rodlike systems in general
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