Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 342590
Title Activation of soluble guanylyl cyclase at the leading edge during Dictyostelium chemotaxis
Author(s) Veltman, D.M.; Roelofs, J.; Engel, R.; Visser, A.J.W.G.; Haastert, P.J.M. van
Source Molecular Biology of the Cell 16 (2005)2. - ISSN 1059-1524 - p. 976 - 983.
DOI https://doi.org/10.1091/mbc.E04-08-0701
Department(s) Biochemistry
EPS-1
Publication type Refereed Article in a scientific journal
Publication year 2005
Keyword(s) adenylyl-cyclase - discoideum - protein - cgmp - cells - transduction - receptor - pathway
Abstract Dictyostelium contains two guanylyl cyclases, GCA, a 12-transmembrane enzyme, and sGC, a homologue of mammalian soluble adenylyl cyclase. sGC provides nearly all chemoattractant-stimulated cGMP formation and is essential for efficient chemotaxis toward cAMP. We show that in resting cells the major fraction of the sGC-GFP fusion protein localizes to the cytosol, and a small fraction is associated to the cell cortex. With the artificial substrate Mn 2+/GTP, sGC activity and protein exhibit a similar distribution between soluble and particulate fraction of cell lysates. However, with the physiological substrate Mg2+/GTP, sGC in the cytosol is nearly inactive, whereas the particulate enzyme shows high enzyme activity. Reconstitution experiments reveal that inactive cytosolic sGC acquires catalytic activity with Mg2+/GTP upon association to the membrane. Stimulation of cells with cAMP results in a twofold increase of membrane-localized sGC-GFP, which is accompanied by an increase of the membrane-associated, guanylyl cyclase activity. In a cAMP gradient, sGC-GFP localizes to the anterior cell cortex, suggesting that in chemotacting cells, sGC is activated at the leading edge of the cell.
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