Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 342755
Title Microarray analysis reveals expression regulation of Wnt antagonists in differentiating osteoblasts
Author(s) Vaes, B.L.T.; Dechering, K.J.; Someren, P. van; Hendriks, J.M.A.; Ven, C.J.J.M. van de; Feijen, A.; Mummery, C.L.; Reinders, M.J.T.; Olijve, W.; Zoelen, E.J.J. van; Steegenga, W.T.
Source Bone 36 (2005)5. - ISSN 8756-3282 - p. 803 - 811.
Department(s) Chair Nutrition Metabolism and Genomics
Publication type Refereed Article in a scientific journal
Publication year 2005
Keyword(s) receptor-related protein-5 - bone morphogenetic protein - growth-factor-beta - signaling pathway - lrp5 gene - mice - density - mouse - transduction - wingless
Abstract Wnt signaling has been implicated in regulating bone formation by controlling osteoblast proliferation and function. Although stabilization of ß-catenin by Wnt has been shown to increase alkaline phosphatase expression and osteoblast differentiation, the precise role of Wnt signaling during the process of osteoblast differentiation is largely unknown. In this study, we used microarray technology to investigate expression regulation of Wnt signaling components during in vitro osteoblast differentiation. Expression was analyzed during bone morphogenetic protein 2 (BMP2)-induced osteoblast differentiation of murine C2C12 and MC3T3 cells and data were compared with expression in BMP2-treated NIH3T3 fibroblasts. During osteoblast differentiation, particularly strong expression regulation of the Wnt antagonists Sfrp2 (secreted frizzled related protein 2) and Wif1 (Wnt inhibitory factor 1) was observed in the late phase of differentiation. In situ expression analysis in murine tail vertebrae supported Wif1 expression during late phase bone cell differentiation, since Wif1 was found to be expressed in vivo in trabecular, but not in cortical bone. We further analyzed the effects of continuous activation of Wnt signaling by lithium chloride and observed that osteoblast differentiation was reduced, as measured by expression of osteoblast marker genes encoding alkaline phosphatase, osteocalcin, and osterix, as well as by the amount of calcium release. Taken together, our data indicate that endogenous expression of Wnt antagonists by osteoblasts provides a negative Wnt feedback loop which is essential in controlling osteoblast maturation
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