Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 343247
Title Single biosensor immunoassay for the detection of five aminoglycosides in reconstituted skimmed milk
Author(s) Haasnoot, W.; Cazemier, G.; Koets, M.; Amerongen, A. van
Source Analytica Chimica Acta 488 (2003)1. - ISSN 0003-2670 - p. 53 - 60.
DOI https://doi.org/10.1016/S0003-2670(03)00628-7
Department(s) RIKILT - Business Unit Safety & Health
AFSG Food Quality
AFSG Biobased Products
Publication type Refereed Article in a scientific journal
Publication year 2003
Keyword(s) performance liquid-chromatography - (dihydro)streptomycin residues - optical immunobiosensor - antibiotic residues - raw-milk - streptomycin - gentamicin - assay
Abstract The application of an optical biosensor (Biacore 3000), with four flow channels (Fcs), in combination with a mixture of four specific antibodies resulted in a competitive inhibition biosensor immunoassay (BIA) for the simultaneous detection of the five relevant aminoglycosides in reconstituted skimmed milk. Four aminoglycosides (gentamicin, neomycine, kanamycin and a streptomycin derivative) were immobilised onto the sensor surface of a biosensor chip (CM5) in the four Fcs of the biosensor system by amine coupling. In the Biacore, milk (reconstituted from skimmed milk powder) was 10 times diluted with a mixture of the four specific antibodies and injected through the four serially connected Fcs (1 min at a flow rate of 20 ¿l min¿1). The responses measured just prior to the injection (20 ¿l at a flow rate of 20 ¿l min¿1) of the regeneration solution (0.2 M NaOH + 20% acetonitril) were indicative for the presence or absence of the aminoglycosides in reconstituted milk. The limits of detection were between 15 and 60 ng ml¿1, which was far below the maximum residue limits (MRLs) (varying from 100 to 500 ng ml¿1) and the total run time between samples was 7 min.
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