Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 34376
Title Increased resistance to 14 alpha-demethylase inhibitors (DMIs) in Aspergillus niger by coexpression of the Penicillium itulicum eburicol 14 alpha-demethylase (cyp51) and the A-niger cytochromeP450 reductase (cprA) genes
Author(s) Brink, J.M. van den; Nistelrooy, J.G.M. van; Waard, M.A. de; Hondel, C.A.M.J.J. van den; Gorcom, R.F.M. van
Source Journal of Biotechnology 49 (1996). - ISSN 0168-1656 - p. 13 - 18.
Department(s) Laboratory of Phytopathology
Publication type Refereed Article in a scientific journal
Publication year 1996
Abstract In this paper we describe the effects of over-expression of the Penicillium italicum gene encoding eburicol 14α-demethylase (cyp51), in Aspergillus niger strains with one or multiple copies of the gene encoding cytochrome P450 reductase (cprA), on the eburicol 14α-demethylase activity. Eburicol 14α-demethylase activity was determined by measuring the resistance of transformants against some eburicol 14α-demethylase inhibitors (DMIs). DMIs are widely used as fungicides in crop protection and human and veterinarian health care. DMI resistance in a transformant overexpressing both CPR and CYP51 was increased 5–30-fold compared to DMI resistance in the wild type strain, depending on the test compound used. Resistance in this strain was approximately 2–5-fold increased compared to DMI resistance in a transformant that was overexpressing the cyp51 gene but had only the wild type copy of the cprA gene and approximately 3–12-fold increased compared to a strain overexpressing the cprA gene (and having only the wild type copy of the cyp51 gene). These results show the importance of CPR overexpression for increasing cytochrome P450 activities in filamentous fungi.
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