Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 344930
Title Evaluation of crucial factors for implementing shed-microspore culture of Indonesian hot pepper (Capsicum annuum L.) cultivars
Author(s) Supena, E.D.J.; Muswita, W.; Suharsono, S.; Custers, J.B.M.
Source Scientia Horticulturae 107 (2006)3. - ISSN 0304-4238 - p. 226 - 232.
Department(s) PRI Bioscience
Laboratory of Plant Breeding
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) triticum-aestivum l. - anther culture - antibiotic timentin - chloroplast number - plant-regeneration - tissue-cultures - colchicine - cell - embryogenesis - haploids
Abstract A shed-microspore culture protocol was developed in Wageningen for producing doubled haploid plants in several genotypes of Indonesian hot pepper (Capsicum annuum L.). For transfer of technology to Indonesia, three factors were studied that appeared crucial for successful implementation in practice. First, application in the culture medium of a combination of the antibiotics timentin and rifampicin at the concentrations of 200 and 10 mg/l, respectively, prevented bacterial contamination from the donor explants. Second, in vitro application of colchicine (100 ¿M) during the first week of culture was highly effective in increasing the percentage of doubled haploid plants. Third, a comparative analysis of the ploidy level of plants regenerated from shed-microspore-derived embryos using chloroplast counts in guard cells of leaf stomata and flow cytometric measurement of leaf nuclear DNA content, revealed that the first procedure is a reliable and an easy to use method for ploidy determination with hot pepper
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