Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 346390
Title Isolation of protoplasts and culture and regeneration into plants in Alstroemeria
Author(s) Kim, J.B.; Bergervoet-van Deelen, J.E.M.; Raemakers, C.J.J.M.; Jacobsen, E.; Visser, R.G.F.
Source In Vitro Cellular and Developmental Biology. Plant 41 (2005)4. - ISSN 1054-5476 - p. 505 - 510.
DOI https://doi.org/10.1079/IVP2005672
Department(s) Laboratory of Plant Breeding
EPS-4
Publication type Refereed Article in a scientific journal
Publication year 2005
Keyword(s) somaclonal variation - cell-cultures - growth - callus - rice
Abstract An efficient system for the regeneration of plants from protoplasts was developed in Alstroemeria. Friable embryogenic callus (FEC) proved to be the best source for protoplast isolation and culture when compared with leaf tissue and compact embryogenic callus. Protoplast isolation was most efficient when FEC was cultured under vacuum for 5 min in an enzyme solution consisting of 4% cellulase, 0.5% Driselase and 0.2% Macerozyme, followed by culture for 12¿16 h in the dark at 24°C. Cell wall formation and colony formation were better in a liquid medium than on a semi-solid agarose medium. Micro-calluses were formed after 4 wk of culture. Ninety percent of the micro-calluses developed into FEC after 12 wk of culture on proliferation medium. FEC cultures produced somatic embryos on a regeneration medium and half of these somatic embryos developed shoots. Protoplast-derived plants showed more somaclonal variation than vegetatively propagated control plants
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