Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 346584
Title Microbial diversity, nitrogen loss and grass production after addition of Effective Micro-organisms® (EM) to slurry manure
Author(s) Vliet, P.C.J. van; Bloem, J.; Goede, R.G.M. de
Source Applied Soil Ecology 32 (2006)2. - ISSN 0929-1393 - p. 188 - 198.
DOI https://doi.org/10.1016/j.apsoil.2005.07.001
Department(s) Chair Soil Biology and Biological Soil Quality
Soil Science Centre
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) gel-electrophoresis analysis - 16s ribosomal-rna - bacteria - soil - communities - survival
Abstract In this study, we used a DNA fingerprinting technique (PCR-DGGE) to investigate if bacteria present in an activated EM suspension (EM-A) were able to maintain or reach significant relative abundances after addition to slurry manure. We also investigated effects of the addition of EM-A to slurry manure on nitrogen losses from the manure and grass biomass production after application of the manure as organic fertilizer. EM® and Agri-mest® were tested in a factorial set-up so that the factors could be distinguished. Effective Organisms (EM) is a mixture of several effective, disease-depressing micro-organisms. Agri-mest contains energized minerals, which will influence biochemical processes occurring in the manure. Different EM-stocks, and EM-Active suspensions prepared by different users showed large variation in bacterial community structure and, thus, low reproducibility. Through the addition of Agri-mest to the slurry manure, loss of inorganic nitrogen from the slurry manure appeared to be reduced. Moreover, after application of such Agri-mest slurry as an organic fertilizer, nitrogen uptake by grass was reduced. Addition of EM-A had no measurable effects on the bacterial diversity and the chemical composition of the slurry manure. In a pot experiment, also no effects of EM on nitrogen uptake and grass biomass production were recorded.
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