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Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 346929
Title Selective estrogenicity in orally exposed transgenic male mice of compounds known to be estrogenic in vitro
Author(s) Veld, M.G.R. ter; Saag, P.T. van der; Rietjens, I.M.C.M.; Murk, A.J.
Source In: Abstracts of the Dutch toxicology days, 13th-14th June 2006 - p. 174 (1) - 174 (1).
Department(s) Sub-department of Toxicology
Publication type Abstract in scientific journal or proceedings
Publication year 2006
Abstract Transgenic reporter gene mice carrying a 3xERE-tataluc- insulated construct were exposed to compounds found to be estrogenic in vitro. Transgenic male mice, chosen for their relative low background levels of circulating estrogens, were fed a phyto-estrogen free diet. They were housed individually and exposed orally via custard (0.5 ml/day) to either 0.3 and 1 mg/kg 17- estradiol dipropionate (EP), 10 and 50 mg/kg bisphenol A (BPA), 10 and 50 mg/kg nonylphenol (NP), 30 and 100 mg/kg di-isoheptyl phthalate (DIHP), 30 and 100 mg/kg di (2-ethylhexyl) phthalate (DEHP), 30 and 100 mg/kg di (2-ethylhexyl) adipate (DEHA), 5 and 50 mg/kg p,p -dichlorodiphenyl-dichloroethylene (DDE) and 1.6 and 16.6 mg/kg quercetin or solvent control. Fourteen hours later the mice were sacrificed and tissues including liver, pituitary, brain, testes and bone were isolated and frozen immediately at -80 ¿C. Maximal luciferase activity, normalized for protein, was induced by 1 mg/kg EP in the liver (5400-fold), while this was 2-times lower at 0.3 mg EP/kg. Several compounds that were estrogenic in vitro were not estrogenic in vivo. However, tissue specific modulation of luciferase expression was found for some compounds, especially BPA induced luciferase in the liver and kidneys. Nonylphenol slightly induced luciferase in the liver and kidneys. Relative few compounds were estrogenic in vivo. In vitro the studied compounds are at least 104 less estrogenic than E2, this might explain the relative small amount of estrogenic compounds in vivo, as relative low concentrations were tested compared to our in vitro study
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