Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 347044
Title Detection of extracellular factor-positive Streptococcus suis serotype 2 strains in tonsillar swabs of live sows by PCR
Author(s) Swildens, B.; Wisselink, H.J.; Engel, B.; Smith, H.E.; Nielen, M.; Verheijden, J.H.; Stegeman, J.A.
Source Veterinary Microbiology 109 (2005)3-4. - ISSN 0378-1135 - p. 223 - 228.
Department(s) ASG Infectieziekten
Publication type Refereed Article in a scientific journal
Publication year 2005
Keyword(s) palatine tonsils - released protein - diagnostic-tests - pigs - type-2 - prevalence - specificity - sensitivity - validation - state
Abstract In this study, the sensitivity (Se) and specificity (Sp) of a PCR for the detection of EF-positive Streptococcus suis serotype 2 strains in tonsillar swabs of live sows were assessed. We sampled 471 sows originating from four farrow-to-finish farms by tonsillar swabbing and collected their tonsils after slaughter. On these specimens, a PCR, a bacterial examination (BE) or both were performed for the detection of EF-positive S. suis serotype 2 strains. Swab-PCR, Tonsil-PCR and Tonsil-BE were regarded as three integral tests. A Bayesian approach was used to calculate the Se and Sp of the tests. Se and Sp for Swab-PCR were 0.63 (95% credibility interval ) and 0.96 (), respectively. Values for Se and Sp of Tonsil-PCR amounted to 0.88 () and 0.94 (), respectively. For Tonsil-BE, Se was 0.65 () and Sp was 0.97 (). Repetition of the swabbing procedure after 10 min resulted in a higher Se 0.85 () than the Se of the first-Swab-PCR. Repetition of the PCR on the same sample did not result in any significant changes in the outcome of the analysis
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