Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 347318
Title The integration of recombination and physical maps in a large-genome monocot using haploid genome analysis in a trihybrid allium population
Author(s) Khrustaleva, L.I.; Melo, P. de; Heusden, A.W. van; Kik, C.
Source Genetics 169 (2005)3. - ISSN 0016-6731 - p. 1673 - 1685.
Department(s) PRI Biodiversity and Breeding
Laboratory of Plant Breeding
Centrum voor Genetische Bronnen Nederland
Publication type Refereed Article in a scientific journal
Publication year 2005
Keyword(s) in-situ hybridization - gene-rich region - meiotic recombination - cytogenetic map - linkage maps - chromosome structure - sequence-analysis - a1-sh2 interval - dna methylation - maize genome
Abstract Integrated mapping in large-genome monocots has been carried out on a limited number of species. Furthermore, integrated maps are difficult to construct for these species due to, among other reasons, the specific plant populations needed. To fill these gaps, Alliums were chosen as target species and a new strategy for constructing suitable populations was developed. This strategy involves the use of trihybrid genotypes in which only one homeolog of a chromosome pair is recombinant due to interspecific recombination. We used genotypes from a trihybrid Allium cepa x (A. roylei x A. fistulosum) population. Recombinant chromosomes 5 and 8 from the interspecific parent were analyzed using genomic in situ hybridization visualization of recombination points and the physical positions of recombination were integrated into AFLP linkage maps of both chromosomes. The integrated maps showed that in Alliums recombination predominantly occurs in the proximal half of chromosome arms and that 57.9% of PstI/MseI markers are located in close proximity to the centromeric region, suggesting the presence of genes in this region. These findings are different from data obtained on cereals, where recombination rate and gene density tends to be higher in distal regions
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