Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 347443
Title Differences in common bean rhizobial populations associated with soil tillage management in southern Brazil
Author(s) Kaschuk, G.; Hungria, M.; Santos, J.C.P.; Berton-Junior, J.F.
Source Soil & Tillage Research 87 (2006)2. - ISSN 0167-1987 - p. 205 - 217.
DOI https://doi.org/10.1016/j.still.2005.03.008
Department(s) Plant Production Systems
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) polymerase-chain-reaction - phaseolus-vulgaris l. - microbial biomass - no-tillage - conventional tillage - genetic diversity - strains - systems - crop - nodulation
Abstract Progressive adoption of no-tillage (NT) agriculture in the tropics is finally reversing physical, chemical, and biological erosion of soil and in Brazil, an estimated 19 Mha are now devoted to NT. Common bean (Phaseolus vulgaris L.) is a main component of Brazilian agriculture, and enhancement of yields has been achieved under NT as a result of mitigation of environmental stresses, resulting in higher N2 fixation. However, the effects of NT on rhizobial diversity are poorly understood. This study evaluated rhizobial diversity in soils planted to common bean under NT or conventional tillage (CT) systems that were compared with natural grassland used for grazing, in the State of Santa Catarina, southern Brazil. Genetic diversity was assessed by the amplification of the DNA by PCR with specific primers (BOX-PCR) and by RFLP-PCR analyses of the 16S rDNA region. A high level of diversity was observed among strains from all three systems, such that the similarity in the clustering analysis of BOX-PCR products ranged from 36% under natural grassland to only 23% for CT strains. High polymorphism was confirmed in the RFLP-PCR analysis; forty-seven different profiles were obtained, none sharing high similarity with the profiles of reference species of common bean rhizobia. These results indicate that other tropical rhizobial species remain to be described. Genetic diversity was higher among the NT than the CT rhizobial strains, especially when the RFLP-PCR profiles were considered. Genetic diversity in the natural grassland was lower than in the cropped systems, possibly due to absence of the host plant and stubble burning in winter. Average yields in the area under NT (e.g. common bean, approximately 1500 kg ha¿1) have been about 30% higher than under CT, therefore high rhizobial diversity may be a parameter indicative of superior soil quality.
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