Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

    We have a manual that explains all the features 

Record number 347449
Title An internal control for the diagnosis of Crown Gall by PCR
Author(s) Cubero, J.; Wolf, J.M. van der; Beckhoven, J.R.C.M. van; Lopez, M.M.
Source Journal of Microbiological Methods 51 (2002)3. - ISSN 0167-7012 - p. 387 - 392.
Department(s) PRI Bioscience
Biointeracties and Plant Health
Publication type Refereed Article in a scientific journal
Publication year 2002
Keyword(s) polymerase-chain-reaction - phytopathogenic agrobacterium strains - plant tumors - tumefaciens - radiobacter - localization - bacteremia - extraction - disease - tissues
Abstract The addition of an internal control (IC) at the appropriate concentration enables recognize of false negatives in the detection of Agrobacterium tumefaciens and improves the reliability of PCR for crown gall diagnosis. Co-amplification of the IC and target sequence from A. tumefaciens ensures the attainment of at least one amplification product in every PCR reaction if the DNA extracted is of high enough quality to be amplified.
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