Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 347984
Title Development of a generic PCR detection of deoxynivalenol- and nivalenol-chemotypes of Fusarium graminearum
Author(s) Li, H.P.; Wu, A.B.; Zhao, C.S.; Scholten, O.E.; Löffler, H.J.M.; Liao, Y.C.
Source FEMS Microbiology Letters 243 (2005)2. - ISSN 0378-1097 - p. 505 - 511.
DOI https://doi.org/10.1016/j.femsle.2005.01.015
Department(s) PRI Biodiversity and Breeding
PRI Management
Publication type Refereed Article in a scientific journal
Publication year 2005
Keyword(s) gibberella-zeae - trichothecene biosynthesis - natural occurrence - mycotoxins - wheat - identification - sporotrichioides - quantification - chromatography - polymorphism
Abstract Based on the intergenic sequences of Tri5¿Tri6 genes involved in the mycotoxin pathways of Fusarium species, a generic PCR assay was developed to detect a 300 bp fragment of deoxynivalenol (DON)-chemotypes and a 360 bp sequence of nivalenol (NIV)- chemotypes of Fusarium graminearum. Mycotoxin chemotypes identified by the PCR assays were confirmed by the chemical analyses of HPLC or GC/MS. Further analysis of 364 F. graminearum isolates from 12 provinces of China showed that 310 were DON-chemotypes and 54 were NIV-chemotypes. Sequence analyses revealed that DON-chemotypes display more variations than NIV-chemotypes. This PCR assay could be used to detect mycotoxin-producing Fusarium-species and may thus help to develop strategies to avoid or reduce mycotoxin contamination of cereals. Also this assay may provide useful alternatives to antibody-based mycotoxin tests
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