Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 348180
Title Open reading frame 132 of Helicoverpa armigera nucleopolyhedrovirus encodes a functional per os infectivity factor (PIF-2)
Author(s) Fang, M.; Nie, Y.; Wang, Q.; Deng, F.; Wang, R.; Wang, H.; Vlak, J.M.; Chen, X.; Hu, Z.H.
Source Journal of General Virology 87 (2006)9. - ISSN 0022-1317 - p. 2563 - 2569.
DOI https://doi.org/10.1099/vir.0.81788-0
Department(s) Laboratory of Virology
PE&RC
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) occlusion-derived virus - single-nucleocapsid nucleopolyhedrovirus - autographa-californica nucleopolyhedrovirus - nuclear polyhedrosis-virus - heliothis-virescens larvae - envelope protein p74 - multiple nucleopolyhedrovirus - orgyia-pseudotsugata - nucleotide-s
Abstract Open reading frame 132 (Ha132) of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) is a homologue of per os infectivity factor 2 (pif-2) of Spodoptera exigua multiple nucleopolyhedrovirus. Sequence analysis indicated that Ha132 encoded a protein of 383 aa with a predicted molecular mass of 44.5 kDa. Alignment of HA132 and its baculovirus homologues revealed that HA132 was highly conserved among baculoviruses, with 14 absolutely conserved cysteine residues. RT-PCR indicated that Ha132 was first transcribed at 24 h post-infection. Western blot analysis showed that a 43 kDa band was detectable in HearNPV-infected HzAM1 cells from 36 h post-infection. Western blots also indicated that HA132 was a component of the occlusion-derived virus, but not of budded virus. Deletion of Ha132 from HearNPV abolished per os infectivity, but had no effect on the infectivity of the budded virus phenotype
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