Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 349073
Title Agroinfection-based high throughput screening reveals specific recognition of INF elicitins in Solanum
Author(s) Vleeshouwers, V.G.A.A.; Driesprong, J.D.; Kamphuis, L.; Torto-Alalibo, T.; Slot, A. van 't; Govers, F.; Visser, R.G.F.; Jacobsen, E.; Kamoun, S.
Source Molecular Plant Pathology 7 (2006)6. - ISSN 1464-6722 - p. 499 - 510.
DOI https://doi.org/10.1111/j.1364-3703.2006.00355.x
Department(s) Laboratory of Plant Breeding
Laboratory of Phytopathology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) broad-spectrum resistance - plant-disease resistance - late blight resistance - phytophthora-infestans - hypersensitive response - innate immunity - flagellin perception - defense responses - raphanus-sativus - cell-death
Abstract We adapted and optimized the use of the Agrobacterium tumefaciens binary PVX expression system (PVX agroinfection) to screen Solanum plants for response to pathogen elicitors and applied the assay to identify a total of 11 clones of Solanum huancabambense and Solanum microdontum, out of 31 species tested, that respond to the elicitins INF1, INF2A and INF2B of Phytophthora infestans. Prior to this study, response to INF elicitins was only known in Nicotiana spp. within the Solanaceae. The identified S. huancabambense and S. microdontum clones also exhibited hypersensitivity-like cell death following infiltration with purified recombinant INF1, INF2A and INF2B, thereby validating the screening protocol. Comparison of INF elicitin activity revealed that Nicotiana plants responded to significantly lower concentrations than Solanum, suggesting variable levels of sensitivity to INF elicitins. We exploited natural variation in response to INF elicitins in the identified Solanum accessions to evaluate the relationship between INF recognition and late blight resistance. Interestingly, several INF-responsive Solanum plants were susceptible to P. infestans. Also, an S. microdontum × Solanum tuberosum (potato) population that segregates for INF response was generated but failed to identify a measurable contribution of INF response to resistance. These results suggest that in Solanum, INF elicitins are recognized as general elicitors and do not have a measurable contribution to disease resistance
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