Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 349411
Title Antisense down-regulation of strawberry endo-beta (1,4)-glucanase genes does not prevent fruit softening during ripening
Author(s) Palomer, X.; Llop-Tous, I.; Vendrell, M.; Krens, F.A.; Schaart, J.G.; Boone, M.J.; Valk, H.C.P.M. van der; Salentijn, E.M.J.
Source Plant Science 171 (2006)5. - ISSN 0168-9452 - p. 640 - 646.
Department(s) PRI Biodiversity and Breeding
AFSG Biobased Products
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) cell-wall polysaccharides - transgenic tomato fruit - fragaria x ananassa - molecular characterization - pectate lyase - higher-plants - expression - endo-1,4-beta-glucanase - endo-beta-1,4-glucanase - suppression
Abstract Strawberry (Fragaria × ananassa Duch.) fruit softening during ripening is associated with the overlapping presence of two divergent endo-ß-(1,4)-glucanases (EC; EGases), Cel1 and Cel2. Antisense down-regulation of both genes was performed to assess the precise role of these endo-ß-(1,4)-glucanases on strawberry fruit softening. Constant down-regulation of cel1 expression throughout ripening was obtained, which was accompanied by reduced Cel1 protein accumulation. However, diminution of the Cel1 protein level together with a reduction of the total EGase activity to 40% of the control level did not affect fruit firmness, thus suggesting that Cel1 protein is not the major determinant of fruit softening during ripening. On the other hand, no significant reduction of Cel2 protein accumulation was found in any of the Cel2 transgenic or Cel1/Cel2 double-transgenic lines obtained. The difficulties encountered to yield a strawberry line with significant reduction of cel2 expression suggest that this gene might be playing a pivotal role on fruit development prior to ripening, thus accounting for the lack of Cel2 protein down-regulation observed
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