Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 350483
Title Carrot arabinogalactan proteins are interlinked with pectins.
Author(s) Immerzeel, P.; Eppink, M.M.; Vries, S.C. de; Schols, H.A.; Voragen, A.G.J.
Source Physiologia Plantarum 128 (2006)1. - ISSN 0031-9317 - p. 18 - 28.
Department(s) Biochemistry
Food Chemistry Group
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) cell-wall polysaccharides - daucus-carota - hairy regions - arabidopsis - suspension - plants - substances - culture - family - pulp
Abstract Cell wall extracts from a carrot cell culture and tap roots were obtained by sequential extraction with water, EDTA buffer solution and cold sodium hydroxide solution. Arabinogalactan proteins (AGPs) were isolated from the extracts and from the medium of the cell culture and analysed for their molecular weight distribution and carbohydrate composition. Copper ions were used to separate the Yariv positive fractions into AGP fractions with a high and a low level of galacturonic acid (GalA). The GalA rich AGP fractions were incubated with pectin methylesterase and polygalacturonase. This enzyme incubation released GalA fragments from the AGP fractions as monitored by HPAEC and MALDI-TOF MS. At least part of carrot AGPs from the medium and cell walls may be covalently linked to pectin containing a homogalacturonan structural element.
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