Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 351306
Title Multiple virus resistance at a high frequency using a single transgene construct
Author(s) Bucher, E.C.; Lohuis, H.; Poppel, P.J. van; Dimitriadou, C.; Goldbach, R.W.; Prins, M.W.
Source Journal of General Virology 87 (2006)12. - ISSN 0022-1317 - p. 3697 - 3701.
DOI http://dx.doi.org/10.1099/vir.0.82276-0
Department(s) Laboratory of Virology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) rna-mediated resistance - spotted wilt virus - tospovirus resistance - interfering rnas - plants - sequence - length
Abstract RNA silencing is a natural antiviral defence in plants, which can be exploited in transgenic plants for preprogramming virus recognition and ensuring enhanced resistance. By arranging viral transgenes as inverted repeats it is thus possible to obtain strong repression of incoming viruses. Due to the high sequence specificity of RNA silencing, this technology has hitherto been limited to the targeting of single viruses. Here it is shown that efficient simultaneous targeting of four different tospoviruses can be achieved by using a single small transgene based on the production of minimal sized chimaeric cassettes. Due to simultaneous RNA silencing, as demonstrated by specific siRNA accumulation, the transgenic expression of these cassettes rendered up to 82% of the transformed plant lines heritably resistant against all four viruses. Thus RNA silencing can be further improved for high frequency multiple virus resistance by combining small RNA fragments from a series of target viruses
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