Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 351681
Title Incorporated sarcolemmal fish oil fatty acids shorten pig ventricular action potentials
Author(s) Verkerk, A.O.; Ginneken, A.C.G. van; Berecki, G.; Ruijter, H.M. den; Schumacher, C.A.; Veldkamp, M.W.; Baartscheer, A.; Casini, S.; Opthof, T.; Hovenier, R.; Fiolet, J.W.T.; Zock, P.L.; Coronel, R.
Source Cardiovascular Research 70 (2006)3. - ISSN 0008-6363 - p. 509 - 520.
Department(s) RIKILT - Business Unit Safety & Health
Chair Nutrition and Health over the Lifecourse
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) heart-failure - dietary supplementation - chloride current - guinea-pig - myocytes - exchange - adult - arrhythmias - inhibition - ischemia
Abstract Background: Omega-3 polyunsaturated fatty acids (W-PUFAs) from fish oil reduce the risk of sudden death presumably by preventing life-threatening arrhythmias. Acutely administered omega 3-PUFAs modulate the activity of several cardiac ion channels, but the chronic effects of a diet enriched with fish oil leading to omega 3-PUFA-incorporation into the sarcolemma on membrane currents are unknown. Methods: Pigs received a diet either rich in W-PUFAs or in omega 9-fatty acids for 8 weeks. Ventricular myocytes (VMs) were isolated and used for patch-clamp studies. Results: omega 3-VMs contained higher amounts of omega 3-PUFAs and had a shorter action potential (AP) with a more negative plateau than control VM. In omega 3 VMs, L-type Ca2+ current (I-Ca,I-L) and Na+-Ca2+ exchange current (I-NCX) were reduced by approximately 20% and 60%, respectively, and inward rectifier K+ current (I-KI) and slow delayed rectifier K+ current (I-Ks) were increased by approximately 50% and 70%, respectively, compared to control. Densities of rapid delayed rectifier K+ current, Ca2+-activated Cl- current, and Na+ current (I-Na) were unchanged, although voltage-dependence of IN,, inactivation was more negative in omega 3 VMs. Conclusions: A fish oil diet increases omega 3-PUFA content in the ventricular sarcolemma, decreases I-Ca,I-L and I-NCX, and increases I-KI and I-Ks, resulting in AP shortening. Incorporation of W-PUFAs in the sarcolemma may have consequences for arrhythmias independent of circulating omega 3-PUFAs. (c) 2006 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.
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