Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 352075
Title Changes in gene expresssion during male meiosis in Petunia hybrida
Author(s) Cnudde, F.; Hedatale, V.; Jong, J.H.S.G.M. de; Pierson, E.S.; Rainey, D.Y.; Zabeau, M.; Weterings, K.; Gerats, T.; Peters, J.L.
Source Chromosome Research 14 (2006)8. - ISSN 0967-3849 - p. 919 - 932.
Department(s) Laboratory of Genetics
Publication type Refereed Article in a scientific journal
Publication year 2006
Keyword(s) genome-wide expression - arabidopsis-thaliana - chromosome synapsis - meiotic prophase - transcriptional program - spermatogenic cells - spindle checkpoint - fission yeast - homolog - protein
Abstract We analyzed changes in gene expression during male meiosis in Petunia by combining the meiotic staging of pollen mother cells from a single anther with cDNA-AFLP transcript profiling of mRNA from the synchronously developing sister anthers. The transcript profiling experiments focused on the identification of genes with a modulated expression profile during meiosis, while premeiotic archesporial cells and postmeiotic microspores served as a reference. About 8000 transcript tags, estimated at 30% of the total transcriptome, were generated, of which around 6% exhibited a modulated gene expression pattern at meiosis. Cluster analysis revealed a transcriptional cascade that coincides with the initiation and progression through all stages of the two meiotic divisions. Fragments that exhibited high expression specifically during meiosis I were characterized further by sequencing; 90 out of the 293 sequenced fragments showed homology with known genes, belonging to a wide range of gene classes, including previously characterized meiotic genes. In-situ hybridization experiments were performed to determine the spatial expression pattern for five selected transcript tags. Its concurrence with cDNA-AFLP transcript profiles indicates that this is an excellent approach to study genes involved in specialized processes such as meiosis. Our data set provides the potential to unravel unique meiotic genes that are as yet elusive to reverse genetics approaches
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