Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 35542
Title Degradation of apple fruit xyloglucan by endoglucanase.
Author(s) Vincken, J.P.; Beldman, G.; Niessen, W.M.A.; Voragen, A.G.J.
Source Carbohydrate Polymers 29 (1996). - ISSN 0144-8617 - p. 75 - 85.
Department(s) Food Chemistry and Microbiology
Publication type Refereed Article in a scientific journal
Publication year 1996
Abstract A purified, alkali-extractable apple fruit xyloglucan (APfxg) was treated by endoglucanase (endoIV) from Trichoderma viride. The degradation products were fractionated by size-exclusion chromatography on BioGel P-2; the pentamer to dodecamer fractions were further fractionated by semi-preparative high-performance anion-exchange chromatography. The purified oligosaccharides were characterized by monosaccharide analysis, mass spectrometry and degradation with Driselase. Based on these data, tentative structures were proposed for most products. Apple xyloglucan is composed of a diversity of repeating units. Next to the major oligosaccharides XXXG, an octamer, XXFG and XLFG (Renard, C.M.G.C., Lomax, J.A. & Boon, J.J. (1992). Carbohydr. Res., 232, 303-320), XG, XXG, FG and two new fucose-containing oligosaccharide building blocks (hexasaccharide and dodecasaccharide) were found. Larger xyloglucan fragments were obtained by partial degradation of APfxg by endoIV, and treatment of apple cell wall material by endol. These fragments were predominantly composed of fucose-containing oligosaccharides. Our results show that both endoIV and endoI are hindered by fucosylated side chains.
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