Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

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Record number 355626
Title An NB-LRR protein required for HR signalling mediated by both extra- and intracellular resistance proteins
Author(s) Gabriëls, S.H.E.J.; Vossen, J.H.; Ekengren, S.K.; Ooijen, G. van; Abd-El-Haliem, A.M.; Berg, G.C.M. van den; Rainey, D.Y.; Martin, G.B.; Takken, F.L.W.; Wit, P.J.G.M. de; Joosten, M.H.A.J.
Source The Plant Journal 50 (2007)1. - ISSN 0960-7412 - p. 14 - 28.
DOI https://doi.org/10.1111/j.1365-313X.2007.03027.x
Department(s) Laboratory of Phytopathology
EPS-2
Publication type Refereed Article in a scientific journal
Publication year 2007
Keyword(s) plant-disease resistance - tobacco-mosaic-virus - cell-death - hypersensitive response - cladosporium-fulvum - functional-analysis - confers resistance - dna interactions - gene-products - arc domain
Abstract Tomato (Solanum lycopersicum) Cf resistance genes confer hypersensitive response (HR)-associated resistance to strains of the pathogenic fungus Cladosporium fulvum that express the matching avirulence (Avr) gene. Previously, we identified an Avr4-responsive tomato (ART) gene that is required for Cf-4/Avr4-induced HR in Nicotiana benthamiana as demonstrated by virus-induced gene silencing (VIGS). The gene encodes a CC-NB-LRR type resistance (R) protein analogue that we have designated NRC1 (NB-LRR protein required for HR-associated cell death 1). Here we describe that knock-down of NRC1 in tomato not only affects the Cf-4/Avr4-induced HR but also compromises Cf-4-mediated resistance to C. fulvum. In addition, VIGS using NRC1 in N. benthamiana revealed that this protein is also required for the HR induced by the R proteins Cf-9, LeEix, Pto, Rx and Mi. Transient expression of NRC1D481V, which encodes a constitutively active NRC1 mutant protein, triggers an elicitor-independent HR. Subsequently, we transiently expressed this auto-activating protein in N. benthamiana silenced for genes known to be involved in HR signalling, thereby allowing NRC1 to be positioned in an HR signalling pathway. We found that NRC1 requires RAR1 and SGT1 to be functional, whereas it does not require NDR1 and EDS1. As the Cf-4 protein requires EDS1 for its function, we hypothesize that NRC1 functions downstream of EDS1. We also found that NRC1 acts upstream of a MAP kinase pathway. We conclude that Cf-mediated resistance signalling requires a downstream NB-LRR protein
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