Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 35672
Title Glycoalkaloids selectively permeabilize cholesterol containing biomembranes.
Author(s) Keukens, E.A.J.; Vrije, T. de; Jansen, L.A.M.; Boer, H. de; Janssen, M.; Kroon, A.I.P.M. de; Jongen, W.M.F.; Kruijff, B. de
Source Biochimica et biophysica acta-protein structure and molecular enzymology 1279 (1996). - ISSN 0167-4838 - p. 243 - 250.
Department(s) Agrotechnological Research Institute
Integrated Food Science and Food Physics
Publication type Refereed Article in a scientific journal
Publication year 1996
Abstract The effects of the glycoalkaloids α-solanine, α-chaconine and α-tomatine on different cell types were studied in order to investigate the membrane action of these compounds. Hemolysis of erythrocytes was compared to 6-carboxyfluorescein leakage from both ghosts and erythrocyte lipid vesicles, whereas leakage of enzymes from mitochondria and the apical and baso-lateral side of Caco-2 cells was determined. Furthermore, the effects of glycoalkaloids on the gap-junctional communication between Caco-2 cells was studied. From these experiments, it was found that glycoalkaloids specifically induced membrane disruptive effects of cholesterol containing membranes as was previously reported in model membrane studies. In addition, α-chaconine was found to selectively decrease gap-junctional intercellular communication. Furthermore, the glycoalkaloids were more potent in permeabilizing the outer membrane of mitochondria compared to digitonin at the low concentrations used.
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