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Record number 35672
Title Glycoalkaloids selectively permeabilize cholesterol containing biomembranes.
Author(s) Keukens, E.A.J.; Vrije, T. de; Jansen, L.A.M.; Boer, H. de; Janssen, M.; Kroon, A.I.P.M. de; Jongen, W.M.F.; Kruijff, B. de
Source Biochimica et biophysica acta-protein structure and molecular enzymology 1279 (1996). - ISSN 0167-4838 - p. 243 - 250.
Department(s) Agrotechnological Research Institute
Integrated Food Science and Food Physics
Publication type Refereed Article in a scientific journal
Publication year 1996
Abstract The effects of the glycoalkaloids α-solanine, α-chaconine and α-tomatine on different cell types were studied in order to investigate the membrane action of these compounds. Hemolysis of erythrocytes was compared to 6-carboxyfluorescein leakage from both ghosts and erythrocyte lipid vesicles, whereas leakage of enzymes from mitochondria and the apical and baso-lateral side of Caco-2 cells was determined. Furthermore, the effects of glycoalkaloids on the gap-junctional communication between Caco-2 cells was studied. From these experiments, it was found that glycoalkaloids specifically induced membrane disruptive effects of cholesterol containing membranes as was previously reported in model membrane studies. In addition, α-chaconine was found to selectively decrease gap-junctional intercellular communication. Furthermore, the glycoalkaloids were more potent in permeabilizing the outer membrane of mitochondria compared to digitonin at the low concentrations used.
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