Staff Publications

Staff Publications

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    'Staff publications' is the digital repository of Wageningen University & Research

    'Staff publications' contains references to publications authored by Wageningen University staff from 1976 onward.

    Publications authored by the staff of the Research Institutes are available from 1995 onwards.

    Full text documents are added when available. The database is updated daily and currently holds about 240,000 items, of which 72,000 in open access.

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Record number 35746
Title Design of aqueous two-phase systems supporting animal cell growth: a first step toward extractive bioconversions.
Author(s) Zijlstra, G.M.; Gooijer, C.D. de; Pol, L.A. van der; Tramper, J.
Source Enzyme and Microbial Technology 19 (1996). - ISSN 0141-0229 - p. 2 - 8.
DOI http://dx.doi.org/10.1016/0141-0229(95)00173-5
Department(s) Sub-department of Food and Bioprocess Engineering
VLAG
Publication type Refereed Article in a scientific journal
Publication year 1996
Abstract The design of aqueous two-phase systems (ATPSs) which support the long-term growth of animal cells is described in this paper. It was found that the increase in osmolality caused by the ATPS-forming polymers could be compensated by reducing the NaCl concentration of the culture medium. Cell growth was possible in culture media containing up to 0.025 g g-1 PEG or 0.15 g g-1 dextran. In ATPSs of PEG 35,000; dextran 40,000; and culture medium; the hybridoma cells partitioned to the PEG-lean phase. In two of these ATPSs, hybridoma cells were successfully cultured over a period of more than two months. The Mab product, however, partitioned along with the cells in the lower phase, but preliminary experiments using PEG ligands showed improved Mab partitioning. The design of aqueous two-phase systems (ATPSs) which support the long-term growth of animal cells is described in this paper. It was found that the increase in osmolality caused by the ATPS-forming polymers could be compensated by reducing the NaCl concentration of the culture medium. Cell growth was possible in culture media containing up to 0.025 g g-1 PEG or 0.15 g g-1 dextran. In ATPSs of PEG 35,000; dextran 40,000; and culture medium; the hybridoma cells partitioned to the PEG-lean phase. In two of these ATPSs, hybridoma cells were successfully cultured over a period of more than two months. The Mab product, however, partitioned along with the cells in the lower phase, but preliminary experiments using PEG ligands showed improved Mab partitioning.
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